机构：[1]The National Key Clinic Specialty, the Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, P. R. China[2]Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, Xi’an, P. R. China[3]Department of Oncology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, P. R. China四川省人民医院[4]Department of Laboratory Medicine, Tongchuan People’s Hospital, Tongchuan, P. R. China[5]Clinical Research Centre, Nanfang Hospital of Southern Medical University, Guangzhou, P. R. China
Objectives: This study is to investigate if polymerase I and transcript release factor (PTRF) acts as a modulator in glioblastoma (GBM) chemoresistance. Methods: Multidrug resistant (MDR) GBM cell line U251AR was established by exposing the U251 cell line to imatinib. The 2D-DIGE and MALDI-TOF/TOF-MS were performed on U251 and U251AR cell lines to screen MDR-related proteins. The expression of PTRF was determined by Western blot and quantitative RT-PCR analyses. Results: When compared with the parental U251 cells, expression of 21 proteins was significantly altered in U251AR cells. Among the 21 differentially expressed proteins, the expression of PTRF was up-regulated by 2.14 folds in U251AR cells when compared with that in the parental U251 cells. Knockdown of PTRF in GBM cell lines significantly increased chemosensitivity of cells to various chemical drugs and decreased the expression levels of caveolin1, a major structural component of caveolae. Expression levels of PTRF and caveolin1 were significantly up-regulated in the relapsed GBM patients. The mRNA level of PTRF and caveolin1 showed a positive correlation in the same GBM specimens. Conclusions: Our results indicate that PTRF acts as a modulator in GBM chemoresistance.
基金：
National Nature Science Foundation of China (81041068, 30971183, 81372691), the Funds for Key Sci-Tech Research
Projects of Guangdong (2009B030801230), Nature Science Foundation of Guangdong Province, China (S2011010004065).
第一作者机构：[1]The National Key Clinic Specialty, the Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, P. R. China[2]Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, Xi’an, P. R. China
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推荐引用方式(GB/T 7714)：
Wang Xin,Liu Tianzhu,Bai Yifeng,et al.Polymerase I and Transcript Release Factor Acts As an Essential Modulator of Glioblastoma Chemoresistance[J].PLOS ONE.2014,9(4):doi:10.1371/journal.pone.0093439.
APA：
Wang, Xin,Liu, Tianzhu,Bai, Yifeng,Liao, Hongzhan,Qiu, Shengcong...&Guo, Hongbo.(2014).Polymerase I and Transcript Release Factor Acts As an Essential Modulator of Glioblastoma Chemoresistance.PLOS ONE,9,(4)
MLA：
Wang, Xin,et al."Polymerase I and Transcript Release Factor Acts As an Essential Modulator of Glioblastoma Chemoresistance".PLOS ONE 9..4(2014)
