To evaluate the effect of preoperative thyroid functional parameters and thyroid autoantibodies on aggressive clinicopathologic features and lymph node metastasis (LNM) of differentiated thyroid cancer patients. Four hundred twenty consecutive patients with initial surgery were enrolled from July 2010 to July 2015. The associations between aggressive clinicopathologic and LNM factors and thyroid functional & autoantibodies parameters were analyzed. Higher levels of TSH, TGAb or TMAb were found in patients with tumor size >= 1 cm (all P< 0.05), especially when TSH >= 2.5 ulU/ml (P= 0.03) and TGAb >= 1 (P= 0.01). Higher levels of TSH and TGAb and lower levels of T3 and T4 were found in patients with capsular invasion (all P< 0.05), particularly when TSH >= 2.5ulU/ml (P= 0.03) and TGAb >= 1 (P= 0.005). The patients with multifocality had higher TAbs level (TAbs> 1). Higher level of TSH was also found in patients with central LNM (P= 0.001) and lateral LNM (P= 0.002), especially with TSH >= 2.5ulU/ml (P= 0.003 and P= 0.03). TGAb level was also found higher in patients with central LNM (P= 0.02) and lateral LNM (P= 0.01), especially with TGAb >= 1 (P< 0.05 and P= 0.01). Higher level of TMAb was found in patients with lateral LNM (P< 0.05). Moreover, multivariable analysis revealed that only TGAb was an independently predictive factor for primary tumor size >= 1cm (P= 0.01); and TSH level (P= 0.01) and TGAb >= 1 (P< 0.05) were associated independently with central LNM. Thus, TSH level and TGAb >= 1 were significantly independent predictors for central LNM, and might help make the decision of central neck dissection.