Aberrant microRNAs (miRNAs) contribute to metastasis of various cancer types, including melanoma. miR-542-3p has been characterized as a tumor suppressor in several cancers. However, the exact expression patterns of miR-542-3p and the precise molecular mechanisms underlying its role in melanoma require further exploration. In the current study, we demonstrated that miR-542-3p is significantly downregulated in melanoma cell lines and clinical specimens. Exogenous expression of miR-542-3p resulted in marked inhibition of melanoma cell migration, invasion and epithelial-mesenchymal transition (EMT) in vitro and lung metastasis in vivo. The proto-oncogene serine/threonine protein kinase, PIM1, was identified as a direct target of miR-542-3p using luciferase reporter assay, qRT-PCR and western blot analyses. Overexpression of PIM1 partially rescued miR-542-3p-mediated suppression of cell migration, invasion and EMT. Our results collectively indicate that miR-542-3p serves as a metastasis suppressor in melanoma, supporting its utility as a promising therapeutic candidate. (C) 2016 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81101231]
第一作者机构：[1]Institute of Dermatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China
通讯作者：
通讯机构：[1]Institute of Dermatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China [*1]Institute of Dermatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, No. 32, Western 2nd Section, 1st Ring Road, Chengdu, Sichuan, China.
推荐引用方式(GB/T 7714)：
Rang Zhen,Yang Ge,Wang You-wei,et al.miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,474(2):315-320.doi:10.1016/j.bbrc.2016.04.093.
APA：
Rang, Zhen,Yang, Ge,Wang, You-wei&Cui, Fan.(2016).miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,474,(2)
MLA：
Rang, Zhen,et al."miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 474..2(2016):315-320
