机构:[1]Department of General Medicine, Sichuan Cancer Hospital, Chengdu 610041, China四川省肿瘤医院[2]Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu 610041, China四川省肿瘤医院[3]Department of Nephrology, Ya’an People’s Hospital, Ya’an 625000, China
Background. MicroRNAs have recently been verified as useful diagnostic biomarkers in various diseases. In this study, we investigated whether miR-217 is a useful diagnostic biomarker and the possible pathological mechanism of miR-217 in this disease. Methods. Patients with focal segmental glomerulosclerosis (FSGS), membranous nephropathy (MN), and diabetic nephropathy (DN) and control patients were enrolled in this study. The miR-217 inhibitor and mimics were transfected into human podocyte cells to investigate the pathological mechanism of miR-217 in this disease. Relevant indicators were detected and tested. Results. Compared with control patients, miR-217 was significantly downregulated and TNFSF11 was significantly upregulated inMN. Then, miR-217 had obvious separation between patients with MN and control patients, with an AUC of 0.941, a cutoff value of <750.0 copies/ul, and sensitivity and specificity of 88.9% and 75.9%. In addition, the TNFSF11 was confirmed to be the target gene of miR-217. Finally, in in vitro experiments, the upregulation of miR-217 could decrease the expression of TNFSF11 and not induce human podocyte cells apoptosis; however, the downregulation of miR-217 could bring about an opposite change. Conclusions. miR-217 is a useful diagnostic biomarker and is involved in human podocyte cells apoptosis via targeting TNFSF11 inmembranous nephropathy.
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外文
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出版当年[2017]版:
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学:研究与实验
最新[2023]版:
无
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出版当年[2017]版:
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构:[1]Department of General Medicine, Sichuan Cancer Hospital, Chengdu 610041, China
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推荐引用方式(GB/T 7714):
Jing Li,Bin Liu,Hen Xue,et al.miR-217 Is a Useful Diagnostic Biomarker and Regulates Human Podocyte Cells Apoptosis via Targeting TNFSF11 in Membranous Nephropathy[J].BIOMED RESEARCH INTERNATIONAL.2017,2017:doi:10.1155/2017/2168767.
APA:
Jing Li,Bin Liu,Hen Xue,Qiao Qiao Zhou&Ling Peng.(2017).miR-217 Is a Useful Diagnostic Biomarker and Regulates Human Podocyte Cells Apoptosis via Targeting TNFSF11 in Membranous Nephropathy.BIOMED RESEARCH INTERNATIONAL,2017,
MLA:
Jing Li,et al."miR-217 Is a Useful Diagnostic Biomarker and Regulates Human Podocyte Cells Apoptosis via Targeting TNFSF11 in Membranous Nephropathy".BIOMED RESEARCH INTERNATIONAL 2017.(2017)