机构：[1]Department of stomatology, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.四川省人民医院四川省肿瘤医院[2]Department of Head and Neck Surgery, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.外科中心头颈外科中心四川省人民医院四川省肿瘤医院头颈外科[3]State Key Laboratory of Oral Diseases, Department of Cleft lip and Palate Surgery, West China Hospital of Stomatology, Sichuan University, Sichuan, China.
Normal cell cycle progression and proliferation of palatal mesenchymal cells are important for palatal development. As targets of miR-17-92, E2F transcription factors family has been suggested to induce the transcription of miR-17-92 in several cell types. In the present study, we sought to investigate whether this negative feedback loop exists in mouse PMCs and what the function of this negative feedback loop would be in palatal mesenchymal cells. Using GeneMANIA, we revealed that the most important function of experimentally verified targets of miR-17-92 is cell cycle regulation. E2F1 and E2F3, but not E2F2, were extensively expressed in mouse palate. Over-expression of E2F1 significantly increased the expression of all the members of miR-17-92. After increased by E2F1, miR-17 and miR-20a may negatively target E2F1, and thereby prevent the cells from excessive proliferation. We suggest that the negative feedback loop between E2F1 and miR-17-92 may contribute to palatal development by regulating the proliferation and cell cycle of palatal mesenchymal cells.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81200771, 81302375, 81672690]; Department of Science and Technology of Sichuan Province [2017JQ0040]
第一作者机构：[1]Department of stomatology, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.[2]Department of Head and Neck Surgery, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of stomatology, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.[2]Department of Head and Neck Surgery, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Sichuan, China.
推荐引用方式(GB/T 7714)：
Li Ling,Shi Bing,Chen Jin,et al.An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells[J].SCIENTIFIC REPORTS.2017,7:doi:10.1038/s41598-017-05479-7.
