机构：[1]Department of Pharmacy,Sichuan Provincial Hospital for Women and Children, Women and Children’s Hospital of Chengdu Medical College[2]Key Laboratory of Reproductive Medicine,Sichuan Provincial Hospital for Women and Children, Women and Children’s Hospital of Chengdu Medical College[3]Department of Pharmacy, Sichuan Cancer Hospital & Institution, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China临床药学部临床药学部四川省人民医院四川省肿瘤医院
In this study, the viability and apoptosis of ovarian cancer cell line OVCAR-3 were assessed using MTT and flow cytometry analysis. GFP-tagged LC3 plasmid transfection was utilized to demonstrate the occurrence of autophagy. The expression of HIF-1 alpha, Beclin1, LC3 and beta-actin were determined with Western blot analysis. We found that hypoxia could inhibit cisplatin induced apoptosis in OVCAR-3 cells and enhance the chemoresistance to cisplatin. Furthermore, GFP-tagged LC3 plasmid transfection and western blot were used to demonstrate that hypoxia induced chemoresistance of OVCAR-3 cells to cisplatin is related to HIF-1 alpha-induced autophagy. All these findings suggest that the cisplatin resistance of ovarian cancer cells is associated with HIF-1 alpha-induced autophagy.
基金：
This work was funded by the National Natural Science
Foundation of China (No. 81703922), Sichuan Science and Technology Program
(2017JY0303), Basic Research Projects of Education Department of Sichuan Province
(18ZB0164 and 18ZB0240) and Research Projects of Chengdu Medical College
(CYZ17-24).
第一作者机构：[1]Department of Pharmacy,Sichuan Provincial Hospital for Women and Children, Women and Children’s Hospital of Chengdu Medical College[2]Key Laboratory of Reproductive Medicine,Sichuan Provincial Hospital for Women and Children, Women and Children’s Hospital of Chengdu Medical College
通讯作者：
通讯机构：[3]Department of Pharmacy, Sichuan Cancer Hospital & Institution, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China[*1]Sichuan Cancer Hospital & Institution, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
推荐引用方式(GB/T 7714)：
FANGYI LONG,WEIXIN LIU,PING JIA,et al.HIF-1 alpha-induced autophagy contributes to cisplatin resistance in ovarian cancer cells[J].PHARMAZIE.2018,73(9):533-536.doi:10.1691/ph.2018.8514.
APA：
FANGYI LONG,WEIXIN LIU,PING JIA,HUAFEI WANG,GANG JIANG&TING WANG.(2018).HIF-1 alpha-induced autophagy contributes to cisplatin resistance in ovarian cancer cells.PHARMAZIE,73,(9)
MLA：
FANGYI LONG,et al."HIF-1 alpha-induced autophagy contributes to cisplatin resistance in ovarian cancer cells".PHARMAZIE 73..9(2018):533-536
