MicroRNA-210 (miR-210) has been associated with hypoxia. It is abnormally expressed in many malignant tumors. In a previous study, Illumine Soledad massively parallel signature sequencing of miRNomes was conducted in non-tumor and hepatocellular carcinoma (hepatoma, HCC) tissues. It was found that expression of miR-210 was abnormally high in HCC. However, expression levels and biological activity levels of miR-210-5p in HCC, as well as its relationship with hypoxia, have not been reported. The current study evaluated human HCC cell lines SMMC-7721, Huh-7, HepG2, and MHCC97H and normal human hepatocytes under normoxia and hypoxia conditions. Results showed that both miR-210-5p and miR-210-3p were highly expressed in hepatoma cells. Relative expression of miR-210-5p was much higher than that of miR-210-3p. In addition, both miR-210-5p and miR-210-3p were significantly upregulated under hypoxia, with relative expression of miR-210-5p still much higher than that of miR-210-3p. Hypoxia also promoted the migration of hepatoma cells in vitro and expression of epithelial-mesenchymal transition (EMT)-related proteins, including N-cadherin, MMP2, Slug, and Twist1. Inhibition of miR-210-5p and miR-210-3p inhibited the migration and expression of EMT-related proteins but had no effects on the proliferation of hepatoma cells. In conclusion, miR-210-5p and miR-210-3p were highly expressed in hepatoma cells and upregulated under hypoxia. Hypoxia-induced migration and EMT in hepatoma cells were regulated by hypoxia-related miR-210-5p and miR-210-3p. However, proliferation of hepatoma cells was not associated with miR-210-5p and miR-210-3p.