机构：[1]Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China华中科技大学同济医学院附属同济医院[2]Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan Province 610041, China四川大学华西医院[3]Wuhan Britain-China School, Wuhan, Hubei 430030, China[4]Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China华中科技大学同济医学院附属同济医院
Aim. To further investigate the mechanism behind the antitumor properties of berberine regarding lipid metabolism. Methods. Cell viability, proliferation, and apoptosis assays were performed to determine the antigrowth effects of berberine in vitro. Ectopic xenograft models in Balb/c nude mice were established to determine the antitumor effects of berberine in vivo. Results. Berberine inhibited cell viability and proliferation of MGC803 human gastric cancer cell lines in a time- and dose-dependent manner. Berberine induced apoptosis of MGC803 and increased the apoptotic rate with higher doses. Berberine induced the accumulation of fatty acid of MGC803 and suppressed the protein expression of FABPs and PPAR alpha. The FABP inhibitor BMS309403 recapitulated the effects of berberine on MGC803 cells. In the xenograft model, berberine significantly decreased the tumor volume and tumor weight and induced apoptosis in tumor tissues. Berberine significantly elevated the fatty acid content and inhibited the expression of FABPs and PPAR alpha in the MGC803 xenograft models. Conclusion. Berberine exerted anticancer effects on human gastric cancer both in vitro and in vivo by inducing apoptosis, which was due to the reduced protein expression of FABPs and the accumulation of fatty acid.
基金：
National Natural Science
Foundation of China (nos. 81673757 and 81573787).
第一作者机构：[1]Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China
通讯作者：
推荐引用方式(GB/T 7714)：
Li Lingli,Peng Ze,Hu Qian,et al.Berberine Suppressed Tumor Growth through Regulating Fatty Acid Metabolism and Triggering Cell Apoptosis via Targeting FABPs[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2020,2020:doi:10.1155/2020/6195050.
APA：
Li Lingli,Peng Ze,Hu Qian,Xu Lijun,Zou Xin...&Yi Ping.(2020).Berberine Suppressed Tumor Growth through Regulating Fatty Acid Metabolism and Triggering Cell Apoptosis via Targeting FABPs.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2020,
MLA：
Li Lingli,et al."Berberine Suppressed Tumor Growth through Regulating Fatty Acid Metabolism and Triggering Cell Apoptosis via Targeting FABPs".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2020.(2020)
