机构：[1]Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China四川省人民医院四川省肿瘤医院[2]Department of Radioation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China[3]Department of Thoracic Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Chengdu 610041, China四川大学华西医院
It has been reported that MicroRNAs (miRNAs) play pivotal roles in the occurrence and progression of a variety of cancers. As reported, miR-4295 promotes cell growth and metastasis in a lot of cancers. Nonetheless, the role and molecular mechanism of miR-4295 in HNSCC still remain unknown. In this study, we discovered miR-4295 expression was significantly upregulated in HNSCC tissues and cell lines, which is also associated with the overall survival of patients. Additionally, suppression of miR-4295 significantly inhibited cell proliferation, migration and EMT process in HNSCC. Through Targetscan website, it was predicted that NPTX1 might be a direct target gene of miR-4295. Then, we verified that NPTX1 could directly interact with miR-4295 via luciferase reporter and RNA assays. What's more, we discovered that there was a significantly negative correlation between NPTX1 and miR-4295 expression. It was indicated by further investigation that the effect of miR-4295 suppression on cell proliferation, migration and EMT process in HNSCC can be restored by knockdown of NPTX1 at the same time. Our results suggested that miR-4295 promoted the progression of HNSCC via regulating NPTX1 expression and miR-4295/NPTX1 axis, which may be a new therapeutic strategy for HNSCC.
基金：
This study was supported by Grant agency of the Science and Technology
Department of Sichuan Province (No. 2017HH0096).
第一作者机构：[1]Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China
通讯作者：
推荐引用方式(GB/T 7714)：
Shun Lu,Cheng Zhou,Bingwen Zou,et al.MiR-4295 facilitates cell proliferation and metastasis in head and neck squamous cell carcinoma by targeting NPTX1[J].GENES AND IMMUNITY.2020,21(1):4-12.doi:10.1038/s41435-019-0081-0.
APA：
Shun Lu,Cheng Zhou,Bingwen Zou,Hanyi Zhang&Mei Feng.(2020).MiR-4295 facilitates cell proliferation and metastasis in head and neck squamous cell carcinoma by targeting NPTX1.GENES AND IMMUNITY,21,(1)
MLA：
Shun Lu,et al."MiR-4295 facilitates cell proliferation and metastasis in head and neck squamous cell carcinoma by targeting NPTX1".GENES AND IMMUNITY 21..1(2020):4-12
