H460/MX20 are derived from large cell lung cancer H460 cell line and then transformed into ABCG2-overexpressing cells by mitoxantrone's induction, which are widely used in study of multidrug resistance (MDR) in vitro. To establish and spread the model of H460/MX20 cell xenografts, we investigated whether cell biological characteristics and the MDR phenotype were maintained in vivo model. Our results demonstrated that the cell proliferation, cell cycle, and ABCG2 expression level in xH460/MX20 cells isolated from H460/MX20 cell xenografts were similar to H460/MX20 cells in vitro. Importantly, xH460/MX20 cells exhibited high levels of resistance to ABCG2 substrates such as mitoxantrone and topotecan as H460/MX20 cells did. Furthermore, lapatinib, the inhibitor of ABCG2, potently reversed mitoxantrone-and topotecan-resistance of xH460/MX20 cells. Taken together, these results suggest that H460/MX20 cell xenografts in athymic nude mice still retain their original cytological characteristics and MDR phenotype. Thus, the H460/MX20 cell xenografts model could serve as a sound model in vivo for study on reversal MDR.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China [81473233, 81402503]; Science and Technology Planning Project of Guangdong Province, China [2013B060300010]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]Sun Yat Sen Univ, Ctr Expt Anim, Guangzhou 510080, Guangdong, Peoples R China;
通讯作者：
通讯机构：[1]Sun Yat Sen Univ, Ctr Expt Anim, Guangzhou 510080, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China;[3]Guangdong Esophageal Canc Inst, Guangzhou 510060, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Wei,Chen Zhen,Chen Likun,et al.ABCG2-overexpressing H460/MX20 cell xenografts in athymic nude mice maintained original biochemical and cytological characteristics[J].SCIENTIFIC REPORTS.2017,7:-.doi:10.1038/srep40064.
APA：
Zhang, Wei,Chen, Zhen,Chen, Likun,Wang, Fang,Li, Furong...&Fu, Liwu.(2017).ABCG2-overexpressing H460/MX20 cell xenografts in athymic nude mice maintained original biochemical and cytological characteristics.SCIENTIFIC REPORTS,7,
MLA：
Zhang, Wei,et al."ABCG2-overexpressing H460/MX20 cell xenografts in athymic nude mice maintained original biochemical and cytological characteristics".SCIENTIFIC REPORTS 7.(2017):-
