机构：[1]Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Hunan, Peoples R China[2]Univ South China, Inst Biol Res, Hengyang, Hunan, Peoples R China[3]Univ South China, Canc Res Inst, Hengyang, Hunan, Peoples R China[4]Univ South China, Inst Pharm & Pharmacol, Hengyang, Hunan, Peoples R China
MicroRNAs (miRNAs) are internal, non-coding, and similar to 22nt small RNAs that display cell- and tissue-specific expression. They play important regulatory roles in cell proliferation and chemo-sensitivity. This study focused on tumor-suppressive miR-33b-5p expression as well as its role in gastric cancer. MiR-33b-5p was found low expression in gastric cancer cell lines. Functionally, western blots and the luciferase reporter assay were used to confirm that HMGA2 was the potential target of miR-33b-5p. Next, we used CCK-8 kits to analyze the effect of miR-33b-5p combined chemotherapy drugs on cell inhibition rate, and flow cytometry to analyze cells apoptosis. Colony formation ability was determined by plating at 500 cells per well into six-well plates and culturing for 15d. The results showed that upregulation of miR-33b-5p decreased expression of HMGA2 and inhibited gastric cancer cell growth as well as sensitized gastric cancer cells to chemotherapy drugs. MiR-33b-5p overexpression hindered luciferase activity of HMGA2,3-untranslated region-based reporter construct in 293T cells. These data demonstrate that miR-33b-5p may be a potential therapeutic target for gastric cancer and function as tumor-suppressive miRNA through targeting HMGA2 in gastric cancer.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81372579]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [17C1402]; Provincial Natural Science Foundation of HunanNatural Science Foundation of Hunan Province [2017JJ2226]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|3 区药学
最新[2023]版：
大类|4 区医学
小类|4 区药学
第一作者：
第一作者机构：[1]Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Hunan, Peoples R China[2]Univ South China, Inst Biol Res, Hengyang, Hunan, Peoples R China
通讯作者：
通讯机构：[1]Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Hunan, Peoples R China[3]Univ South China, Canc Res Inst, Hengyang, Hunan, Peoples R China[4]Univ South China, Inst Pharm & Pharmacol, Hengyang, Hunan, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Xiaoyan,Zhao Qian,Yin Huali,et al.MiR-33b-5p sensitizes gastric cancer cells to chemotherapy drugs via inhibiting HMGA2 expression[J].JOURNAL OF DRUG TARGETING.2017,25(7):653-660.doi:10.1080/1061186X.2017.1323220.
APA：
Yang, Xiaoyan,Zhao, Qian,Yin, Huali,Lei, Xiaoyong&Gan, Runliang.(2017).MiR-33b-5p sensitizes gastric cancer cells to chemotherapy drugs via inhibiting HMGA2 expression.JOURNAL OF DRUG TARGETING,25,(7)
MLA：
Yang, Xiaoyan,et al."MiR-33b-5p sensitizes gastric cancer cells to chemotherapy drugs via inhibiting HMGA2 expression".JOURNAL OF DRUG TARGETING 25..7(2017):653-660
