HLA-E inhibitor enhances the killing of neuroblastoma stem cells by co-cultured dendritic cells and cytokine-induced killer cells loaded with membrane-based microparticles
Neuroblastoma stem cells (NSCs) can cause drug resistance and tumor recurrence. This study aimed to enhance the lytic effect of dendritic cells (DCs) co-cultured with cytokine-induced killer (CIK) cells. NSCs were obtained by suspension culture, and DC-CIK cells were loaded with extracted NSC membrane-based microparticles (MMPs) before evaluating the lytic effect of DC-CIK cells on NSCs. After inhibiting the function or expression of human leukocyte antigen-E (HLA-E) in NSCs by anti-HLA-E monoclonal antibody or siRNA, the DC-CIK cell lytic effect on NSCs was re-assessed. NSC nestin expression was high, but glial fibrillary acid protein expression and class III beta-tubulin-1 expression were low. Moreover, NSCs exhibited strong tumorigenic ability in nude mice. Loading DCs with NSC-derived MMPs induced the differentiation of DCs and CIK cells and enhanced the killing of NSCs by DC-CIK cells. Inhibiting the function or expression of HLA-E in NSCs further enhanced the cytolytic capability of DC-CIK cells loaded with NSC-derived MMPs. HLA-E inhibitor can enhance the killing of NSC by DC-CIK cells loaded with NSC-derived MMPs.
基金：
Science and Technology Planning Project of Guangdong Province, China [2013B021800069, 2012B031800460]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|3 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Ctr Canc, Dept Pediat Oncol, 651 Dongfeng Rd East, Guangzhou 510060, Guangdong, Peoples R China;[4]Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China;
通讯作者：
通讯机构：[1]State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Ctr Canc, Dept Pediat Oncol, 651 Dongfeng Rd East, Guangzhou 510060, Guangdong, Peoples R China;[4]Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China;
推荐引用方式(GB/T 7714)：
Zhen Zijun,Yang Kaibin,Ye Litong,et al.HLA-E inhibitor enhances the killing of neuroblastoma stem cells by co-cultured dendritic cells and cytokine-induced killer cells loaded with membrane-based microparticles[J].AMERICAN JOURNAL OF CANCER RESEARCH.2017,7(2):334-345.
APA：
Zhen, Zijun,Yang, Kaibin,Ye, Litong,You, Zhiyao,Chen, Rirong...&He, Youjian.(2017).HLA-E inhibitor enhances the killing of neuroblastoma stem cells by co-cultured dendritic cells and cytokine-induced killer cells loaded with membrane-based microparticles.AMERICAN JOURNAL OF CANCER RESEARCH,7,(2)
MLA：
Zhen, Zijun,et al."HLA-E inhibitor enhances the killing of neuroblastoma stem cells by co-cultured dendritic cells and cytokine-induced killer cells loaded with membrane-based microparticles".AMERICAN JOURNAL OF CANCER RESEARCH 7..2(2017):334-345
