Multiplexed Electrochemical Detection of MiRNAs from Sera of Glioma Patients at Different Stages via the Novel Conjugates of Conducting Magnetic Microbeads and Diblock Oligonucleotide-Modified Gold Nanoparticles
机构：[1]Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China;[2]Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med, Guangzhou 510060, Guangdong, Peoples R China;其他部门华南肿瘤学国家重点实验室中山大学肿瘤防治中心[3]Cent S Univ, Canc Res Inst, Changsha 410013, Hunan, Peoples R China
MicroRNAs (miRNAs) serve as diagnostic and prognostic biomarkers for a wide variety of cancers. Via the novel conjugates of gold nanopartide-coated magnetic microbeads (AuNP-MMBs) and the diblock oligonudeotide (ODN)-modified AuNPs, multiplexed electrochemical assay of miRNAs was performed. The hybridization to target miRNAs leads to the conformational change of the hairpin-structured ODN probes, and the attachment of the diblock ODN-modified AuNPs was achieved. By examining the oxidation peak currents of methylene blue (MB) and ferrocene (Fc) moieties residing on the diblock ODNs, simultaneous quantification of miRNA-182 and miRNA-381 was conducted. The detection signals were significantly enhanced due to the numerous MB and Fc tags on the AuNPs. The proposed assay was highly selective for discriminating miRNAs with similar sequences, and detection limits of 0.20 fM and 0.12 fM for miRNA-182 and miRNA-381, respectively, were achieved. The feasibility of the method for sensitive determination of miRNA-182 and miRNA-381 from serum samples of glioma patients at different stages was demonstrated. The sensing protocol thus holds great potential for early diagnosis and treatment of cancer patients.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21375150, 21575166, 21705166, 81772961]; Chinese National Key Basic Research ProgramNational Basic Research Program of China [2014CB744502]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2017]版：
大类|1 区化学
小类|1 区分析化学
最新[2023]版：
大类|1 区化学
小类|1 区分析化学
第一作者：
第一作者机构：[1]Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China;
通讯作者：
通讯机构：[1]Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China;
推荐引用方式(GB/T 7714)：
Wang Jingrui,Lu Zhixuan,Tang Hailin,et al.Multiplexed Electrochemical Detection of MiRNAs from Sera of Glioma Patients at Different Stages via the Novel Conjugates of Conducting Magnetic Microbeads and Diblock Oligonucleotide-Modified Gold Nanoparticles[J].ANALYTICAL CHEMISTRY.2017,89(20):10834-10840.doi:10.1021/acs.analchem.7b02342.
APA：
Wang, Jingrui,Lu, Zhixuan,Tang, Hailin,Wu, Ling,Wang, Zixiao...&Wang, Jianxiu.(2017).Multiplexed Electrochemical Detection of MiRNAs from Sera of Glioma Patients at Different Stages via the Novel Conjugates of Conducting Magnetic Microbeads and Diblock Oligonucleotide-Modified Gold Nanoparticles.ANALYTICAL CHEMISTRY,89,(20)
MLA：
Wang, Jingrui,et al."Multiplexed Electrochemical Detection of MiRNAs from Sera of Glioma Patients at Different Stages via the Novel Conjugates of Conducting Magnetic Microbeads and Diblock Oligonucleotide-Modified Gold Nanoparticles".ANALYTICAL CHEMISTRY 89..20(2017):10834-10840
化学