Introduction: The extent to which intratumoral heterogeneity of programmed death ligand 1 (PD-L1) expression causes discordance of PD-L1 expression between paired samples remains unclear. Here, PD-L1 status was,compared between whole sections from NSCLCs and the corresponding tissue microarrays (TMAs) serving as surrogate biopsy specimens. Methods: PD-L1 expression was evaluated by 22C3 immunohistochemistry assay on 190 archival surgical specimens and matched to the TMA results. PD-L1 expression was determined by the tumor proportion score (TPS) and classified as TPS lower than 1%, TPS of 1% to 49%, and TPS of 50% or higher. Agreement statistics were used. Results: The percentage of PD-L1 expression on tumor cells differed greatly between individual TMAs and matched surgical specimens. When PD-L1 TPS was adopted, a total of 36 of 190 discordance cases (18.9%) were observed, with a x-value of 0.630 between paired samples. The TMAs underestimated or overestimated PD-L1 status in 19 of 36 (52.8%) and 17 of 36 (47.2%) of the matched surgical specimens, respectively (p = 0.118). The discordance rate was much lower in cases with a PD-L1 TPS lower than 1% compared with in cases with a TPS of 1% to 49% and TPS of 50% or higher (18.4% versus 56.7% and 43.3%, p < 0.001). When a TPS of 50% or higher was used as the cutoff, the discordance rate of PD-L1 TPS less than 50% was further reduced to 7.5%. Such discrepancies were due mainly to intratumoral heterogeneity of PD-L1 expression and nonsignificant association with clinicopathological features. Conclusions: PD-L1 expression in TMAs correlates moderately well with that in the corresponding surgical specimens, indicating that evaluating PD-L1 expression in diagnostic biopsy specimens could be misleading in defining sensitivity to pembrolizumab treatment yet may be reliable as a way to exclude patients with a PD-L1 TPS less than 50% from first-line pembrolizumab treatment. (C) 2017 International Association for the Study of Lung Cancer. Published by Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81372599]; Fujian Provincial Health Systemic Youth Backbone Training Projects [2015-ZQN-ZD-9]; Fujian Province Natural Science FoundationNatural Science Foundation of Fujian Province [2015J01434]
语种：
外文
被引次数：
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中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区肿瘤学2 区呼吸系统
最新[2023]版：
大类|1 区医学
小类|1 区肿瘤学1 区呼吸系统
第一作者：
第一作者机构：[1]Fujian Canc Hosp, Dept Pathol, Fuzhou, Fujian, Peoples R China;[2]Fujian Med Univ, Canc Hosp, Fuzhou 350014, Fujian, Peoples R China;[3]Fujian Prov Key Lab Translat Canc Med, Fuzhou, Fujian, Peoples R China;
通讯作者：
通讯机构：[2]Fujian Med Univ, Canc Hosp, Fuzhou 350014, Fujian, Peoples R China;[4]Fujian Canc Hosp, Dept Thorac Oncol, Fuzhou 350014, Fujian, Peoples R China;
推荐引用方式(GB/T 7714)：
Li Chao,Huang Cheng,Mok Tony S.,et al.Comparison of 22C3 PD-L1 Expression between Surgically Resected Specimens and Paired Tissue Microarrays in Non-Small Cell Lung Cancer[J].JOURNAL OF THORACIC ONCOLOGY.2017,12(10):1536-1543.doi:10.1016/j.jtho.2017.07.015.
APA：
Li, Chao,Huang, Cheng,Mok, Tony S.,Zhuang, Wu,Xu, Haipeng...&Lin, Gen.(2017).Comparison of 22C3 PD-L1 Expression between Surgically Resected Specimens and Paired Tissue Microarrays in Non-Small Cell Lung Cancer.JOURNAL OF THORACIC ONCOLOGY,12,(10)
MLA：
Li, Chao,et al."Comparison of 22C3 PD-L1 Expression between Surgically Resected Specimens and Paired Tissue Microarrays in Non-Small Cell Lung Cancer".JOURNAL OF THORACIC ONCOLOGY 12..10(2017):1536-1543
医学