In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. NDV-infected and starvation-induced chick embryo fibroblasts (CEF) cells showed higher autophagosome formation than mock-infected CEF cells on transmission electron microscopy. The NDV-infected CEF cells showed enhanced conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to LC3-II and degradation of p62/SQSTM1. The diminished conversion of LC3-I to LC3-II and cleaved caspase 3 and poly (ADP-ribose) polymerase (PARP) in ultraviolet-inactivated NDV-infected cells suggested that autophagosome formation was necessary for NDV replication. Inhibition of autophagy by chloroquine (CQ) enhanced apoptosis resulting in increased cleavage of caspase 3 and PARP and AnnexinV/propidium iodide staining. Autophagy induction by rapamycin resulted in upregulation of all autophagy-related genes except Beclin 1, anti-apoptosis factors, and proinflammatory cytokines in the NDV-infected spleen and lung tissues. Subsequently, decreased apoptosis was observed in NDV-infected spleens and lungs than mock-infected organs. The pan-caspase inhibitor ZVAD-FMK promoted conversion of LC3-I to LC3-II, the degradation of p62/SQSTM1, NDV replication and cell viability by inhibiting apoptosis. Our study demonstrates that apoptosis inhibition enhances autophagy and promoted cell survival and NDV replication.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31072139, 31372412]; Science and Technology Projects of Guangdong Province [2012A020800006]; Chinese Special Fund for Agro-scientific Research in the Public Interest [201303033]; Doctoral Fund of Ministry of Education of ChinaMinistry of Education, China [20124404110016]
语种：
外文
被引次数：
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PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区肿瘤学3 区细胞生物学
最新[2023]版：
无
第一作者：
第一作者机构：[1]South China Agr Univ, Coll Vet Med, Key Lab Zoonosis Prevent & Control Guangdong Prov, Guangzhou 510642, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med,Dept Expt Res, Guangzhou 510642, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Anim Vaccine Dev, Guangzhou 510642, Guangdong, Peoples R China;
通讯作者：
通讯机构：[1]South China Agr Univ, Coll Vet Med, Key Lab Zoonosis Prevent & Control Guangdong Prov, Guangzhou 510642, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med,Dept Expt Res, Guangzhou 510642, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Anim Vaccine Dev, Guangzhou 510642, Guangdong, Peoples R China;
推荐引用方式(GB/T 7714)：
Kang Yinfeng,Yuan Runyu,Xiang Bin,et al.Newcastle disease virus-induced autophagy mediates antiapoptotic signaling responses in vitro and in vivo[J].ONCOTARGET.2017,8(43):73981-73993.doi:10.18632/oncotarget.18169.
APA：
Kang, Yinfeng,Yuan, Runyu,Xiang, Bin,Zhao, Xiaqiong,Gao, Pei...&Ren, Tao.(2017).Newcastle disease virus-induced autophagy mediates antiapoptotic signaling responses in vitro and in vivo.ONCOTARGET,8,(43)
MLA：
Kang, Yinfeng,et al."Newcastle disease virus-induced autophagy mediates antiapoptotic signaling responses in vitro and in vivo".ONCOTARGET 8..43(2017):73981-73993
