机构：[1]Division of Digestive Endoscopy, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011内科科室消化内科消化内镜科大连医科大学附属第一医院[2]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101[3]Sun Yat?Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, Guangdong 510060其他部门华南肿瘤学国家重点实验室中山大学肿瘤防治中心[4]The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Collaborative Innovation Center for Cardiovascular Disorders, Beijing Institute of Heart, Lung and Blood Vessel Diseases,Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China临床科室心脏内科中心首都医科大学附属安贞医院
Ring finger protein 8 (RNF8), an FHA/RING domain containing E3 ubiquitin ligase, is critical in supporting genome integrity by facilitating the assembly of multiple DNA repair proteins at DNA lesions following DNA damage. In the present study, a search for novel binding partners of RNF8 was performed using a yeast two-hybrid screening assay, and small ubiquitin-like modifier (SUMO) 2/3 was identified as one of the major RNF8-binding candidates. GST pull-down and immunoprecipitation assays revealed that RNF8 bound directly and noncovalently to SUMO2/3, but not to SUMO1, and that the FHA domain of RNF8 was required for the binding to SUMO2/3. Furthermore, RNF8 co-localized with SUMO2/3 at sites of DNA lesions in response to ionizing radiation, as revealed by immunofluorescence assay. Survival assay indicated that the depletion of RNF8 and SUMO2/3 resulted in decreased cellular resistance to genotoxic stress. These data suggested that the binding of RNF8 to SUMO2/3 promoted the response to DNA damage.
基金：
China Postdoctoral Science Foundation [2015T80145]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|4 区医学：研究与实验4 区肿瘤学
第一作者：
第一作者机构：[1]Division of Digestive Endoscopy, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011[*1]Division of Digestive Endoscopy, The First Affiliated Hospital of Dalian Medical University, 222 Zhong Shan Road, Dalian, Liaoning 116011, P.R. China
通讯作者：
通讯机构：[1]Division of Digestive Endoscopy, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011[*1]Division of Digestive Endoscopy, The First Affiliated Hospital of Dalian Medical University, 222 Zhong Shan Road, Dalian, Liaoning 116011, P.R. China
推荐引用方式(GB/T 7714)：
Liang Lili,Zhang Zhenzhu,Li Jiandong,et al.Direct binding of RNF8 to SUMO2/3 promotes cell survival following DNA damage[J].Molecular medicine reports.2017,16(6):8385-8391.doi:10.3892/mmr.2017.7624.
APA：
Liang, Lili,Zhang, Zhenzhu,Li, Jiandong,Wu, Jiaoxiang,Wang, Likui...&Gao, Shijuan.(2017).Direct binding of RNF8 to SUMO2/3 promotes cell survival following DNA damage.Molecular medicine reports,16,(6)
MLA：
Liang, Lili,et al."Direct binding of RNF8 to SUMO2/3 promotes cell survival following DNA damage".Molecular medicine reports 16..6(2017):8385-8391
