机构：[1]Department of Medical Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, China. lingwenwu@wchscu.cn.四川大学华西医院[2]Department of Radiology, Huaxi MR Research Center (HMRRC), Institute of Radiology and Medical Imaging, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, Sichuan, China. xiaohe.t@wchscu.cn.四川大学华西医院[3]Psychoradiology Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu, Sichuan, China.四川大学华西医院[4]Research Unit of Psychoradiology, Chinese Academy of Medical Sciences, Chengdu, Sichuan, China.[5]Xiamen Key Lab of Psychoradiology and Neuromodulation, Department of Radiology, West China Xiamen Hospital of Sichuan University, Xiamen, Fujian, China.[6]Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[7]Department of Food and Environmental Engineering, Chuzhou Polytechnic, Chuzhou, China.
Mitochondrial viscosity plays a pivotal role in the microenvironment of mitochondria and is closely associated with pathological conditions. As a light-activated treatment for cancer and infections, photodynamic therapy (PDT) relies on reactive oxygen species (ROS) generation by photosensitizers to induce cell apoptosis, and mitochondria are prime targets of PDT. However, current photosensitizers lack real-time feedback on therapeutic efficacy. Herein, we report Mito-Qu and Mito-QuE, two quinolinium-based multifunctional fluorescent probes for mitochondrial targeting and viscosity response based on the D-π-A structure to overcome these limitations. Mito-Qu and Mito-QuE reveal exceptionally large Stokes shifts of 179 nm and 186 nm and exhibit viscosity-sensitive emission via a twisted intramolecular charge transfer (TICT) mechanism, making them robust sensors for monitoring mitochondrial viscosity dynamics. Additional experiments suggest that Mito-Qu acts as a sensitive monitor for mitochondrial viscosity monitoring and self-reporting PDT efficacy via both confocal imaging and FLIM, providing a blueprint for developing next-generation organelle-targeted probes for intracellular microenvironment monitoring.
基金：
This research was financially supported by the Sichuan Provincial
Department of Science and Technology as a project of Central
Guided for Local Science and Technology Development Funds
(2024ZYD0056), the Sichuan Science and Technology Program
(No. 2024YFFK0228), the Chengdu Science and Technology Program (No. 2024-YF05-00110-SN) and the Key Research and Development Program of Science and Technology Department of
Sichuan Province (No. 2023YFS0214)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2025]版：
大类|3 区材料科学
小类|3 区材料科学：生物材料
最新[2025]版：
大类|3 区材料科学
小类|3 区材料科学：生物材料
第一作者：
第一作者机构：[1]Department of Medical Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, China. lingwenwu@wchscu.cn.
共同第一作者：
通讯作者：
通讯机构：[2]Department of Radiology, Huaxi MR Research Center (HMRRC), Institute of Radiology and Medical Imaging, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, Sichuan, China. xiaohe.t@wchscu.cn.[3]Psychoradiology Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu, Sichuan, China.[4]Research Unit of Psychoradiology, Chinese Academy of Medical Sciences, Chengdu, Sichuan, China.[5]Xiamen Key Lab of Psychoradiology and Neuromodulation, Department of Radiology, West China Xiamen Hospital of Sichuan University, Xiamen, Fujian, China.
推荐引用方式(GB/T 7714)：
Ren Youxiang,Su Liping,Tang Xuelin,et al.FLIM-guided self-reporting of PDT efficacy using a mitochondria-targeted, viscosity-sensitive quinolinium probe[J].Journal Of Materials Chemistry. B.2025,doi:10.1039/d5tb01668a.
APA：
Ren Youxiang,Su Liping,Tang Xuelin,Hu Panyi,Cao Jiazhi...&Ling Wenwu.(2025).FLIM-guided self-reporting of PDT efficacy using a mitochondria-targeted, viscosity-sensitive quinolinium probe.Journal Of Materials Chemistry. B,,
MLA：
Ren Youxiang,et al."FLIM-guided self-reporting of PDT efficacy using a mitochondria-targeted, viscosity-sensitive quinolinium probe".Journal Of Materials Chemistry. B .(2025)
