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Bioinspired ruthenium-manganese-oxygen complex for biocatalytic and radiosensitization therapies to eradicate primary and metastatic tumors

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机构: [1]Department of Biotherapy, Cancer Center,West China Hospital, Sichuan University, Chengdu, China. [2]College of Polymer Science and Engineering, State Key Laboratory of Advanced Polymer Materials, Sichuan University, Chengdu, China. [3]College of Biological Engineering, Sichuan University of Science and Engineering, Yibin, China. [4]School of Pharmacy and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao, China. [5]Institute of Chemistry and Biochemistry, Free University of Berlin, Berlin, Germany. [6]Department of Endodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
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Designing efficient, biocompatible radiation-sensitive materials to activate systemic immune responses can maximize tumoricidal effects against malignant tumors. Here, inspired by natural Mn-peroxidase, we propose the de novo design of the RuMn-oxygen complex (MnBTC-Ru) for biocatalytic and radiosensitization therapies to eradicate primary and metastatic tumors. Our results reveal that Mn-organic ligands can enhance the electron density of Ru clusters, thereby optimizing their binding to oxygen species and resulting in high reactive oxygen species and oxygen generation. Accordingly, MnBTC-Ru with radiation can enhance cell membrane and DNA damage, triggering apoptosis though oxidative damage, heightening radiosensitization, and activating CD8+ T cells. When combined with anti-PD-1 therapy, this synergistic approach generates robust systemic antitumor responses in female mice, promoting the abscopal effect and establishing enduring immune memory against tumors, thereby reducing recurrence and metastasis. This design presents superior biocatalytic and radiosensitizing properties, which may provide promising and practical bio-nanotechnology for future treatments on eradicating primary and metastatic tumors.© 2025. The Author(s).

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第一作者机构: [1]Department of Biotherapy, Cancer Center,West China Hospital, Sichuan University, Chengdu, China.
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通讯机构: [2]College of Polymer Science and Engineering, State Key Laboratory of Advanced Polymer Materials, Sichuan University, Chengdu, China. [6]Department of Endodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
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