Hyaluronic acid (HA)-derived hydrogels signify a noticeable development in biomedical uses, especially in cancer treatment and wound repair. Cancer continues to be one of the foremost causes of death globally, with current therapies frequently impeded by lack of specificity, serious side effects, and the emergence of resistance. HA hydrogels, characterized by their distinctive three-dimensional structure, hydrophilic nature, and biocompatibility, create an advanced platform for precise drug delivery, improving therapeutic results while minimizing systemic toxicity. These hydrogels facilitate the regulated release of medications, genes, and various therapeutic substances, enhancing the effectiveness of chemotherapy, radiotherapy, and immunotherapy. Additionally, they can be designed to react to stimuli like pH, light, and magnetic fields, enhancing their therapeutic capabilities. In the process of wound healing, the hydrophilic and porous characteristics of HA hydrogels establish a moist setting that encourages cell growth and aids in tissue restoration. By imitating the extracellular matrix, they promote tissue regeneration, improve angiogenesis, and influence immune reactions. This review examines the various functions of HA-based hydrogels in cancer treatment and wound healing, highlighting their advancement, uses, and ability to change existing therapeutic methods in these important health sectors.Copyright © 2025. Published by Elsevier B.V.