机构：[1]Department of Laboratory Medicine & Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.四川大学华西医院[2]Precision Medicine Translational Research Center, West China Hospital, Sichuan University, Chengdu 610213, Sichuan, China.四川大学华西医院[3]Center for High Altitude Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.四川大学华西医院[4]Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.[5]College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, Shandong, China.
Accurate diagnosis of highly aggressive and deadly tumors is essential for effective treatment and improved patient outcomes, and microRNAs (miRNAs) have emerged as crucial biomarkers for their roles in tumor initiation, progression, and metastasis. Herein, we present an on-site visualization colorimetric assay for tumor-associated miRNAs using ruthenium nanoparticle decorated titanium dioxide nanoribbon (Ru@TiO2) as a peroxidase-like (POD) nanozyme. Remarkably, the Ru@TiO2 nanozyme can catalyze the oxidation of chromogenic substrates through its POD-like activity, which is effectively inhibited by pyrophosphate generated during the rolling circle amplification process, thereby enabling miRNA detection through a visible colorimetric readout. This approach provides a highly sensitive and specificity assay for miRNAs in diluted human serum with a detection limit of 100 pM. It shows great potential for clinical diagnostics and biological research, offering a promising tool for early cancer diagnosis and molecular diagnostics.
基金：
Ministry of Science and Technology of China (Nos. 2023YFC3040500 and 2022YFB3205604), the Fundamental Research Funds for the Central Universities (No. ZYGX2022YGRH002) and the
National Natural Science Foundation of China (Nos.
22072015 and 32201090).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|1 区化学
小类|1 区分析化学
最新[2023]版：
大类|1 区化学
小类|1 区分析化学
第一作者：
第一作者机构：[1]Department of Laboratory Medicine & Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[2]Precision Medicine Translational Research Center, West China Hospital, Sichuan University, Chengdu 610213, Sichuan, China.
通讯作者：
通讯机构：[1]Department of Laboratory Medicine & Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[2]Precision Medicine Translational Research Center, West China Hospital, Sichuan University, Chengdu 610213, Sichuan, China.[3]Center for High Altitude Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[4]Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.[5]College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, Shandong, China.
推荐引用方式(GB/T 7714)：
Zhang Limei,He Xun,Bai Hao,et al.On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO2 as a Peroxidase-like Nanozyme[J].Analytical Chemistry.2024,96(42):16499-16504.doi:10.1021/acs.analchem.4c03922.
APA：
Zhang Limei,He Xun,Bai Hao,Yu Xingzhi,Wu Peilin...&Hu Wenchuang.(2024).On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO2 as a Peroxidase-like Nanozyme.Analytical Chemistry,96,(42)
MLA：
Zhang Limei,et al."On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO2 as a Peroxidase-like Nanozyme".Analytical Chemistry 96..42(2024):16499-16504
化学