详情页 - 四川省肿瘤医院知识库

当前位置：首页 > 详情页

Capture of RNA-binding proteins across mouse tissues using HARD-AP

8| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE ◇ 自然指数

作者：

机构： [1]Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China. [2]National Maize Improvement Center, Frontiers Science Center for Molecular Design Breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China. [3]Sichuan Provincial Key Laboratory for Human Disease Gene Study and the Center for Medical Genetics, Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China. [4]Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China. [5]Shimmer Center, Tianfu Jiangxi Laboratory, Chengdu, Sichuan 641419, China. [6]State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. [7]Department of Urology, Institute of Urology, State Key Laboratory of Biotherapy and Cancer Center, Sichuan University, Chengdu, Sichuan 610041, China. [8]Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. [9]Development and Related Diseases of Women and Children Key Laboratory of Sichuan Province, Chengdu, Sichuan 610041, China.

出处：

DOI：

ISSN：

摘要：

RNA-binding proteins (RBPs) modulate all aspects of RNA metabolism, but a comprehensive picture of RBP expression across tissues is lacking. Here, we describe our development of the method we call HARD-AP that robustly retrieves RBPs and tightly associated RNA regulatory complexes from cultured cells and fresh tissues. We successfully use HARD-AP to establish a comprehensive atlas of RBPs across mouse primary organs. We then systematically map RNA-binding sites of these RBPs using machine learning-based modeling. Notably, the modeling reveals that the LIM domain as an RNA-binding domain in many RBPs. We validate the LIM-domain-only protein Csrp1 as a tissue-dependent RNA binding protein. Taken together, HARD-AP is a powerful approach that can be used to identify RBPomes from any type of sample, allowing comprehensive and physiologically relevant networks of RNA-protein interactions.© 2024. The Author(s).

基金：

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2024]版：

无

最新[2023]版：

大类 | 1 区

综合性期刊

小类 | 1 区综合性期刊

JCR分区：

出版当年[2024]版：

无

最新[2023]版：

Q1 MULTIDISCIPLINARY SCIENCES

影响因子： 14.7 最新[2023版] 16.1 最新五年平均 14.7 出版当年[2023版] 0 出版当年五年平均 14.7 出版前一年[2023版]

第一作者：

第一作者机构： [1]Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China.

共同第一作者：

通讯作者：

通讯机构： [1]Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China. [9]Development and Related Diseases of Women and Children Key Laboratory of Sichuan Province, Chengdu, Sichuan 610041, China.

推荐引用方式(GB/T 7714)：

APA：

MLA：