Integrated metabolomics revealed the photothermal therapy of melanoma by Mo2C nanosheets: toward rehabilitated homeostasis in metabolome combined lipidome
机构：[1]Department of Plastic and Burn Surgery, Laboratory of Clinical Proteomics and Metabolomics, Institutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, P. R. China.四川大学华西医院[2]NHC Key Laboratory of Transplant Engineering and Immunology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[3]Department of Neurosurgery, Sichuan Clinical Medical Research Center for Neurosurgery, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, P. R. China.[4]Metabolomics and Proteomics Technology Platform, West China Hospital, Sichuan University, Chengdu, P. R. China.四川大学华西医院[5]Department of Clinical Research Management, West China Hospital, Sichuan University, Chengdu, 610041, China.四川大学华西医院[6]Department of Mechanical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, P. R. China.[7]Chengdu Research Institute, City University of Hong Kong, Chengdu, P. R. China.[8]Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[9]Laboratory of Mitochondria and Metabolism, Department of Anesthesiology, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院
Melanoma, the most aggressive and life-threatening form of skin cancer, lacks innovative therapeutic approaches and deeper bioinformation. In this study, we developed a photothermal therapy (PTT) based on Mo2C nanosheets to eliminate melanoma while utilizing integrated metabolomics to investigate the metabolic shift of metabolome combined lipidome during PTT at the molecular level. Our results demonstrated that 1 mg ml-1 Mo2C nanosheets could efficiently convert laser energy into heat with a strong and stable photothermal effect (74 ± 0.9 °C within 7 cycles). Furthermore, Mo2C-based PTT led to a rapid decrease in melanoma volume (from 3.299 to 0 cm2) on the sixth day, indicating the effective elimination of melanoma. Subsequent integrated metabolomics analysis revealed significant changes in aqueous metabolites (including organic acids, amino acids, fatty acids, and amines) and lipid classes (including phospholipids, lysophospholipids, and sphingolipids), suggesting that melanoma caused substantial fluctuations in both metabolome and lipidome, while Mo2C-based PTT helped improve amino acid metabolism-related biological events (such as tryptophan metabolism) impaired by melanoma. These findings suggest that Mo2C nanosheets hold significant potential as an effective therapeutic agent for skin tumors, such as melanoma. Moreover, through exploring multidimensional bioinformation, integrated metabolomics technology provides novel insights for studying the metabolic effects of tumors, monitoring the correction of metabolic abnormalities by Mo2C nanosheet therapy, and evaluating the therapeutic effect on tumors.
基金：
This work was supported by the Science and Technology Department
of Sichuan Province (No. 2023YFH0073, 2022YFSY0001,
2023NSFSC1065, 2022YFS0630, 2022NSFSC1236, and
2023NSFSC1538), the National Natural Science Foundation
project (No. 82072780 and 82372825), the 1.3.5 project for
disciplines of excellence, West China Hospital, Sichuan University
(ZYGD18014) and the Post-Doctor Research Project,
West China Hospital, Sichuan University (2020HXBH014,
2021HXBH052).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|3 区医学
小类|3 区材料科学：生物材料
最新[2023]版：
大类|3 区医学
小类|3 区材料科学：生物材料
第一作者：
第一作者机构：[1]Department of Plastic and Burn Surgery, Laboratory of Clinical Proteomics and Metabolomics, Institutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, P. R. China.[2]NHC Key Laboratory of Transplant Engineering and Immunology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Plastic and Burn Surgery, Laboratory of Clinical Proteomics and Metabolomics, Institutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, P. R. China.[2]NHC Key Laboratory of Transplant Engineering and Immunology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
推荐引用方式(GB/T 7714)：
Zhang Dingkun,Wang Ming,Li Yijin,et al.Integrated metabolomics revealed the photothermal therapy of melanoma by Mo2C nanosheets: toward rehabilitated homeostasis in metabolome combined lipidome[J].Journal Of Materials Chemistry. B.2024,12(3):730-741.doi:10.1039/d3tb02123h.
APA：
Zhang Dingkun,Wang Ming,Li Yijin,Liang Ge,Zheng Wen...&Gong Meng.(2024).Integrated metabolomics revealed the photothermal therapy of melanoma by Mo2C nanosheets: toward rehabilitated homeostasis in metabolome combined lipidome.Journal Of Materials Chemistry. B,12,(3)
MLA：
Zhang Dingkun,et al."Integrated metabolomics revealed the photothermal therapy of melanoma by Mo2C nanosheets: toward rehabilitated homeostasis in metabolome combined lipidome".Journal Of Materials Chemistry. B 12..3(2024):730-741
