机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China四川省人民医院四川省肿瘤医院[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China四川省人民医院[3]Department of Oncology, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, China
Tumor-associated tertiary lymphoid structures (TLSs) are ectopic lymphoid formations within tumor tissue, with mainly B and T cell populations forming the organic aggregates. The presence of TLSs in tumors has been strongly associated with patient responsiveness to immunotherapy regimens and improving tumor prognosis. Researchers have been motivated to actively explore TLSs due to their bright clinical application prospects. Various studies have attempted to decipher TLSs regarding their formation mechanism, structural composition, induction generation, predictive markers, and clinical utilization. Meanwhile, the scientific approaches to qualitative and quantitative descriptions are crucial for TLS studies. In terms of detection, hematoxylin and eosin (H&E), multiplex immunohistochemistry (mIHC), multiplex immunofluorescence (mIF), and 12-chemokine gene signature have been the top approved methods. However, no standard methods exist for the quantitative analysis of TLSs, such as absolute TLS count, analysis of TLS constituent cells, structural features, TLS spatial location, density, and maturity. This study reviews the latest research progress on TLS detection and quantification, proposes new directions for TLS assessment, and addresses issues for the quantitative application of TLSs in the clinic.
基金：
This work was supported by the Key Projects of Sichuan
Natural Science Foundation (No. 2022NSFSC0051), the Clinical
Scientist Program of Sichuan Cancer Hospital (No. YB2022003),
and the Chengdu Technology Innovation R&D Project (No.
2021YF0501659SN), China.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|3 区生物学
小类|3 区生物工程与应用微生物4 区生化与分子生物学4 区医学：研究与实验
最新[2023]版：
大类|3 区生物学
小类|3 区生物工程与应用微生物4 区生化与分子生物学4 区医学：研究与实验
JCR分区：
出版当年[2023]版：
Q1BIOCHEMISTRY & MOLECULAR BIOLOGYQ1BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1BIOCHEMISTRY & MOLECULAR BIOLOGYQ1BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China
通讯作者：
通讯机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China[3]Department of Oncology, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, China
推荐引用方式(GB/T 7714)：
Yang Man,Che Yurou,Li Kezhen,et al.Detection and quantitative analysis of tumor-associated tertiary lymphoid structures[J].JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B.2023,24(9):779-795.doi:10.1631/jzus.B2200605.
APA：
Yang Man,Che Yurou,Li Kezhen,Fang Zengyi,Li Simin...&Wang Weidong.(2023).Detection and quantitative analysis of tumor-associated tertiary lymphoid structures.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,24,(9)
MLA：
Yang Man,et al."Detection and quantitative analysis of tumor-associated tertiary lymphoid structures".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 24..9(2023):779-795
