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Association of microRNA-652 expression with radiation response of colorectal cancer: A study from rectal cancer patients in a Swedish trial of preoperative radiotherapy

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机构： [1]Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Hospital and Research Institute, Kelambakkam, Chennai, Tamil Nadu - 603103, India. [2]Department of Oncology and Department of Biomedical and Clinical Sciences, Linkoping University, 581 83 Linkoping, Sweden. [3]Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Sichuan-Chengdu, China. [4]Linkoping University Hospital Oncology Linkoping Sweden. [5]Department of Biotechnology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu - 620024, India. [6]School of Medicine, Department of Medical Sciences, Orebro University, Orebro, Sweden. [7]Department of Oncology-Pathology, Karolinska Institute, 171 77 Solna, Sweden

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Radiotherapy is a standard adjuvant therapy in patients with progressive rectal cancer, but many patients are resistant to radiotherapy, leading to poor prognosis. Our study identified microRNA-652 (miR-652) value on radiotherapy response and outcome in rectal cancer patients.miR-652 expression was determined by qPCR in primary rectal cancer from 48 patients with and 53 patients without radiotherapy. The association of miR-652 with biological factors and the prognosis was examined. The biological function of miR-652 was identified through TCGA and GEPIA database searches. Two human colon cancer cell lines (HCT116 p53+/+ and p53-/-) were used for in vitro study. The molecular interactions of miR-652 and tumor suppressor genes were studied through a computational approach.In RT patients, miR-652 expression was significantly decreased in cancers when compared to non-radiotherapy cases (P=0.002). High miR-652 expression in non-RT patients was with increased apoptosis marker (P=0.036), ATM (P=0.010), and DNp73 expression (P=0.009). High miR-652 expression was related to worse disease-free survival of non-radiotherapy patients, independent of gender, age, tumor stage, and differentiation (P=0.028; HR=7.398, 95% CI 0.217-3.786). The biological functional analysis further identified the prognostic value and potential relationship of miR-652 with apoptosis in rectal cancer. miR-652 expression in cancers was negatively related to WRAP53 expression (P=0.022). After miR-652 inhibition, the estimation of reactive oxygen species, caspase activity, and apoptosis in HCT116 p53+/+ cells was significantly increased compared with HCT116 p53-/- cells after radiation. The results of the molecular docking analysis show that the miR652-CTNNBL1 and miR652-TP53 were highly stable.Our findings suggest the potential value of miR-652 expression as a marker for the prediction of radiation response and clinical outcome in rectal cancer patients.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

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出版当年[2023]版中科院分区表Review为是的期刊

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大类 | 4 区医学

小类 | 4 区遗传学

最新[2023]版

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大类 | 4 区医学

小类 | 4 区遗传学

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第一作者机构： [1]Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Hospital and Research Institute, Kelambakkam, Chennai, Tamil Nadu - 603103, India. [2]Department of Oncology and Department of Biomedical and Clinical Sciences, Linkoping University, 581 83 Linkoping, Sweden.

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