机构：[1]Department of Laboratory Medicine, Med+X Center for Manufacturing, Institute of Urology, Out-Patient Department, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.四川大学华西医院[2]College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China.
Herein, we report a fluorescence strategy for the homogeneous and simultaneous analysis of urine miRNA-375 and miRNA-148a. The target miRNAs in urine bonded the devised dumbbell-shaped "C-Ag+-C" and "T-Hg2+-T" hairpin structures that could trigger cascade enzyme-free amplification. Then, the fluorescent CdTe quantum dots (QDs) and carbon dots (CDs) could selectively recognize Ag+ and Hg2+, to quantify the dual miRNAs concurrently. Under optimized conditions, the linear range was from 0.1 to 1000 fM and the limits of detection (LOD) for dual miRNAs reached 30 and 25 aM, respectively. The practicality was further evaluated with 45 clinical urine samples including prostate cancer (PC) and other patients, and the results were consistent with the clinical polymerase chain reaction (PCR) kit and ultrasonic and pathological findings. The receiver operating characteristic (ROC) curve analysis showed that the estimates of the area under the curve (AUC) were 0.739 for the serum prostate-specific antigen (PSA) and 0.941 for miRNA-375 and 0.946 for miRNA-148a. The sensitivity and specificity reached 75 and 100% for miRNA-375 and 71 and 94% for miRNA-148a, respectively, which was better than serum PSA. This strategy constructed a reliable system for dual miRNA detection in urine samples and proposed new insights into the rapid and noninvasive diagnosis of PC.
基金：
This work was supported by the National Natural Science
Foundation of China (82202638, 81873979, and 81401666),
Sichuan Science and Technology Program (2021YFS0232),
National Key Research and Development Program of China
(2021YFC2009306 and 2021YFC2009300), and National
Clinical Research Center for Geriatrics, West China Hospital,
Sichuan University (Z2023YY002).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|1 区化学
小类|1 区分析化学
最新[2023]版：
大类|1 区化学
小类|1 区分析化学
第一作者：
第一作者机构：[1]Department of Laboratory Medicine, Med+X Center for Manufacturing, Institute of Urology, Out-Patient Department, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Laboratory Medicine, Med+X Center for Manufacturing, Institute of Urology, Out-Patient Department, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.[*1]Department of Laboratory Medicine, Med+X Center for Manufacturing, Institute of Urology, Out-Patient Department, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
推荐引用方式(GB/T 7714)：
Jiang Pengjun,Bai Yunjin,Yan Li,et al.Nanoarchitectonics-Assisted Simultaneous Fluorescence Detection of Urinary Dual miRNAs for Noninvasive Diagnosis of Prostate Cancer[J].Analytical chemistry.2023,doi:10.1021/acs.analchem.3c00701.
APA：
Jiang Pengjun,Bai Yunjin,Yan Li,Feng Pan,Huang Ke...&Chen Piaopiao.(2023).Nanoarchitectonics-Assisted Simultaneous Fluorescence Detection of Urinary Dual miRNAs for Noninvasive Diagnosis of Prostate Cancer.Analytical chemistry,,
MLA：
Jiang Pengjun,et al."Nanoarchitectonics-Assisted Simultaneous Fluorescence Detection of Urinary Dual miRNAs for Noninvasive Diagnosis of Prostate Cancer".Analytical chemistry .(2023)
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