机构：[1]Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan Province, China四川大学华西医院[2]Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin, China[3]Department of Respiratory and Critical Care Medicine, People’s Hospital of Deyang City, Affiliated Hospital of Chengdu College of Medicine, Deyang, Sichuan Province, China[4]Department of Liver Surgery & Liver Transplantation, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Province, China四川大学华西医院[5]Department of General Surgery, the Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, China[6]The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan Province, China[7]Department of Anesthesiology, Huashan Hospital, Fudan University, Shanghai, China[8]Institute of Medical Security for Maritime Rights Protection of Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin, China
There are various clinical treatments for traumatic brain injury, including surgery, drug therapy, and rehabilitation therapy; however, the therapeutic effects are limited. Scaffolds combined with exosomes represent a promising but challenging method for improving the repair of traumatic brain injury. In this study, we determined the ability of a novel 3D-printed collagen/chitosan scaffold loaded with exosomes derived from neural stem cells pretreated with insulin-like growth factor-1 (3D-CC-INExos) to improve traumatic brain injury repair and functional recovery after traumatic brain injury in rats. Composite scaffolds comprising collagen, chitosan, and exosomes derived from neural stem cells pretreated with insulin-like growth factor-1 (INExos) continuously released exosomes for 2 weeks. Transplantation of 3D-CC-INExos scaffolds significantly improved motor and cognitive functions in a rat traumatic brain injury model, as assessed by the Morris water maze test and modified neurological severity scores. In addition, immunofluorescence staining and transmission electron microscopy showed that 3D-CC-INExos implantation significantly improved the recovery of damaged nerve tissue in the injured area. In conclusion, this study suggests that transplanted 3D-CC-INExos scaffolds might provide a potential strategy for the treatment of traumatic brain injury and lay a solid foundation for clinical translation.
基金：
This work was supported by the National Major Scientific and Technological Special Project for Significant New Drugs Development, No. 2019ZX09301-147 (to LXZ).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|2 区医学
小类|3 区细胞生物学3 区神经科学
最新[2023]版：
大类|2 区医学
小类|3 区细胞生物学3 区神经科学
第一作者：
第一作者机构：[1]Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan Province, China[2]Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin, China
共同第一作者：
通讯作者：
通讯机构：[2]Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin, China[8]Institute of Medical Security for Maritime Rights Protection of Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin, China
推荐引用方式(GB/T 7714)：
Liu Xiao-Yin,Feng Yin-He,Feng Qing-Bo,et al.Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury[J].Neural Regeneration Research.2023,18(9):1990-1998.doi:10.4103/1673-5374.366497.
APA：
Liu Xiao-Yin,Feng Yin-He,Feng Qing-Bo,Zhang Jian-Yong,Zhong Lin...&Zhou Liang-Xue.(2023).Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury.Neural Regeneration Research,18,(9)
MLA：
Liu Xiao-Yin,et al."Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury".Neural Regeneration Research 18..9(2023):1990-1998
