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Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

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机构： [1]Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China [2]Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China [3]Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School ofMedicine, Winston Salem, NC 27157, USA

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Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy, respectively. We then detail the establishment of xenograft tumor mouse model and the in vivo detecting of phagocytosis efficiency by flow cytometry and qRT-PCR. This protocol provides a convenient way to assess macrophage-mediated phagocytosis of cancer cells. For complete details on the use and execution of this protocol, please refer to Xu et al.1.Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

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出版当年[2023]版：

Q4 BIOCHEMICAL RESEARCH METHODS

最新[2023]版：

Q4 BIOCHEMICAL RESEARCH METHODS

影响因子： 1.3 最新[2023版] 1.5 最新五年平均 1.3 出版当年[2023版] 1.5 出版当年五年平均 0 出版前一年[2022版]

第一作者：

第一作者机构： [1]Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China [2]Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China [3]Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School ofMedicine, Winston Salem, NC 27157, USA

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通讯作者：

通讯机构： [1]Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China [2]Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China [3]Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School ofMedicine, Winston Salem, NC 27157, USA

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