机构：[1]Department of Chemical Engineering, Imperial College London, South Kensington, London, UK.[2]Department of Surgery and Cancer, Imperial College London, South Kensington, London, UK.[3]West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China[4]The Imperial College Ophthalmic Research Group (ICORG), Imperial College London, London, UK[5]The Western Eye Hospital, Imperial College Healthcare NHS Trust (ICHNT), London, UK[6]Glaucoma and Retinal Neurodegeneration Group, Department of Visual Neuroscience, UCL Institute of Ophthalmology, London, UK
Glaucoma, a ruinous group of eye diseases with progressive degeneration of the optic nerve and vision loss, is the leading cause of irreversible blindness. Accurate and timely diagnosis of glaucoma is critical to promote secondary prevention and early disease-modifying therapies. Reliable, cheap, and rapid tests for measuring disease activities are highly required. Brain-derived neurotrophic factor (BDNF) plays an important role in maintaining the function and survival of the central nervous system. Decreased BDNF levels in tear fluid can be seen in glaucoma patients, which indicates that BDNF can be regarded as a novel biomarker for glaucoma. Conventional ELISA is the standard method to measure the BDNF level, but the multi-step operation and strict storage conditions limit its usage in point-of-care settings. Herein, a one-step and a portable glaucoma detection method was developed based on the lateral flow assay (LFA) to quantify the BDNF concentration in artificial tear fluids. The results of the LFA were analyzed by using a portable and low-cost system consisting of a smartphone camera and a dark readout box fabricated by 3D printing. The concentration of BDNF was quantified by analyzing the colorimetric intensity of the test line and the control line. This assay yields reliable quantitative results from 25 to 300 pg mL-1 with an experimental detection limit of 14.12 pg mL-1. The LFA shows a high selectivity for BDNF and high stability in different pH environments. It can be readily adapted for sensitive and quantitative testing of BDNF in a point-of-care setting. The BDNF LFA strip shows it has great potential to be used in early glaucoma detection.
基金：
A. K. Y. and Y. H. thank the Engineering and Physical
Sciences Research Council (EP/T013567/1). N. J. acknowledges
the National Natural Science Foundation of China (No.
82102182) and the Fundamental Research Funds for the
Central Universities (No. YJ202152).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|1 区工程技术
小类|1 区分析化学1 区仪器仪表1 区生化研究方法2 区化学：综合2 区纳米科技
最新[2023]版：
大类|2 区工程技术
小类|1 区生化研究方法1 区分析化学2 区化学：综合2 区仪器仪表2 区纳米科技
第一作者：
第一作者机构：[1]Department of Chemical Engineering, Imperial College London, South Kensington, London, UK.[2]Department of Surgery and Cancer, Imperial College London, South Kensington, London, UK.
通讯作者：
通讯机构：[2]Department of Surgery and Cancer, Imperial College London, South Kensington, London, UK.[4]The Imperial College Ophthalmic Research Group (ICORG), Imperial College London, London, UK[5]The Western Eye Hospital, Imperial College Healthcare NHS Trust (ICHNT), London, UK[6]Glaucoma and Retinal Neurodegeneration Group, Department of Visual Neuroscience, UCL Institute of Ophthalmology, London, UK
推荐引用方式(GB/T 7714)：
Wu Yue,Hu Yubing,Jiang Nan,et al.Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma[J].Lab on a chip.2022,doi:10.1039/d2lc00431c.
APA：
Wu Yue,Hu Yubing,Jiang Nan,Anantharanjit Rajeevan,Yetisen Ali K&Cordeiro M Francesca.(2022).Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma.Lab on a chip,,
MLA：
Wu Yue,et al."Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma".Lab on a chip .(2022)
工程技术