机构:[1]Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University.四川大学华西医院[2]Department of Neurosurgery, West China Hospital, Sichuan University.四川大学华西医院[3]Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University四川大学华西医院[4] weiyan2018@scu.edu.cn.[4]Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University四川大学华西医院[6] zhenhuashao@scu.edu.cn.
Lysophospholipids (LPLs) are bioactive lipids that include sphingosine 1-phosphate (S1P), lysophosphatidic acid, etc. S1P, a metabolic product of sphingolipids in the cell membrane, is one of the best-characterized LPLs that regulates a variety of cellular physiological responses via signaling pathways mediated by sphingosine 1-phosphate receptors (S1PRs). This implicated that the S1P-S1PRs signaling system is a remarkable potential therapeutic target for disorders, including multiple sclerosis (MS), autoimmune disorders, cancer, inflammation, and even COVID-19. S1PRs, a small subset of the class A G-protein coupled receptor (GPCR) family, are composed of five subtypes: S1PR1, S1PR2, S1PR3, S1PR4, and S1PR5. The lack of detailed structural information, however, impedes the drug discovery targeting S1PRs. Here, we applied the cryo-electron microscopy method to solve the structure of the S1P-S1PRs complex, and elucidated the mechanism of activation, selective drug recognition, and G-protein coupling by using cell-based functional assays. Other lysophospholipid receptors (LPLRs) and GPCRs can also be studied using this strategy.
语种:
外文
PubmedID:
中科院(CAS)分区:
出版当年[2022]版:
大类|4 区综合性期刊
小类|4 区综合性期刊
最新[2025]版:
大类|4 区综合性期刊
小类|4 区综合性期刊
第一作者:
第一作者机构:[1]Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University.
推荐引用方式(GB/T 7714):
Cheng Lin,Su Lantian,Tian Xiaowen,et al.A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors.[J].Journal of visualized experiments : JoVE.2022,(184):doi:10.3791/64054.
APA:
Cheng Lin,Su Lantian,Tian Xiaowen,Xia Fan,Zhao Chang...&Shao Zhenhua.(2022).A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors..Journal of visualized experiments : JoVE,,(184)
MLA:
Cheng Lin,et al."A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors.".Journal of visualized experiments : JoVE ..184(2022)